Answered Essay: An enzyme catalyzed reaction was carried out in a solution containing 0.2M Tris buffer (pK_a

An enzyme catalyzed reaction was carried out in a solution containing 0.2M Tris buffer (pKa 8.0). The pH of the reaction mixture was 7.7 at the start of the experiment. 6. has the same effect on the buffer as the production of an equivalent amount of OH ions). i. ii. ii. What was the ratio of the Tris/ TrisH at the end of the reaction? What was the ratio of Tris (free base) to TrisH (acid form) at the start of the reaction? Write out an equilibrium that shows the relationship between Tris, TrisH, and their interaction with OH iv. What was the final pH of the reaction mixture? HOH2C CH2OH HOH2C CH2OH NH3 Tris TrisH CH2OH CH2OH

An enzyme catalyzed reaction was carried out in a solution containing 0.2M Tris buffer (pK_a = 8.0). The pH of the reaction mixture was 7.7 at the start of the experiment. During the reaction 0.033 mol/l of H^+ were consumed. (Note that the utilization of H^+ ions has the same effect on the buffer as the production of an equivalent amount of OH^- ions). i. What was the ratio of the Tris (free base) to TrisH (acid form) at the start of the reaction? ii. Write out an equilibrium that shows the relationship between Tris, TrisH, and their interaction with OH^- iii. What was the ratio of the Tris/TrisH at the end of the reaction? iv. What was the final pH of the reaction mixture?

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