Answered Essay: Indicate the net charge of arginine at the chosen pH 9.5.

Indicate the net charge of arginine at the chosen pH 9.5.

H2N HN H2N COO

H2N HN H2N COO

Expert Answer

Answer

If pH < pKa, H+ is on. It means that the group is protonated

If it happens to be for -NH2 group, it will have +1 charge.

For -COOH group in this case, there will be no charge

If pH > pKa, H+ is off. It means that the group is de protonated

If it happens to be for -COOH group, it will have -1 charge.

For -NH2 group in this case, there will be no charge

For Arginine:

pKa of acidic part, pKa1 = 2.17

pKa of basic part, pKa2 = 9.04

pKa of side chain, pKa3 = 12.48

It has basic side chain

pH is greater than pKa1. So, acidic part will not have its H+ on

So, negative charge is present because of it

pH is greater than pKa2. So, baisc part will not have H+ on it

So, no charge because of it

pH is less than pKa3 and since it is basic, it will have H+ on it

So, positive charge is present because of it

Hence net charge at pH = 9.5 is 0

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